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pG28B5.0
pG28B5.0
規格:
價格:
編號:B237259
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pG28B5.0
商品貨號
B237259
Designations
pG28B5.0
GenBank Number

U34070

培養基
Species
Homo sapiens, human
Depositors
KG Xanthopoulos
Applications
contains sequence CCAAT/enhancer binding protein (C/EBP), alpha
Vector
Construct size (kb): 8.199999809265137
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-3Zf(+)
Intact vector size: 3.199
Type of vector: phagemid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI
SphI HindIII
Polylinker sites: T7-EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII
PstI SphI HindIII-SP6
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
promoter: lac, SP6, T7
replicon: pMB1, f1
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CEBPA
Gene name: CCAAT/enhancer binding protein (C/EBP), alpha
Contains complete coding sequence?: Y
Chromosome: 19; Localization: 19 q13.1
Tissue: umbilical vein endothelial cells
Type of DNA: genomic
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 5.0
Cross references: DNA Seq. Acc.: U34070
Nucleotides ~700-4000 of the insert correspond to
nucleotides 1-3318 of U34070.
Insert lengths(kb): 5.0
Tissue: umbilical vein endothelial cells
Gene product: CCAAT/enhancer binding protein (C/EBP), alpha [CEBPA]
Target Gene: CCAAT/enhancer binding protein (C/EBP), alpha
Insert Size (kb)
5.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.0, 3.2; HindIII--6.2, 1.5, 0.46; EcoRI--5.6, 2.5.
Insert contains the following restriction sites (approximate kb from the 5' end): BglII--3.0; EcoRI--2.5; EcoRV--3.2; HindIII--3.1, 4.6; NcoI--1.3, 2.3; NotI--1.65, 1.7; PstI--0.7, 1.3, 1.85, 1.9, 4.4; SacI--2.3, 3.9; XbaI--3.5; XhoI--0.2, 2.35.
The probe used for Northern blotting was the 0.7 kb EcoRI/HindIII fragment from the 3' untranslated region of the insert.
References

Antonson P, Xanthopoulos KG. Molecular cloning, sequence, and expression patterns of the human gene encoding CCAAT/enhancer binding protein alpha (C/EBP alpha). Biochem. Biophys. Res. Commun. 215: 106-113, 1995. PubMed: 7575576

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