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<em>Saccharomyces cerevisiae</em> Meyen ex E.C. Hansen

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編號:B237126

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產品名稱

Saccharomyces cerevisiae Meyen ex E.C. Hansen

商品貨號

B237126

Classification

Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae

Strain Designations

EBY100

培養基

Alternate State

Candida robusta Diddens et Lodder

Application

These cells are used in the yeast display method to isolate novel proteins based on various biological functions.

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format

frozen

Storage Conditions

Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section

Genotype

MATa AGA1::GAL1-AGA1::URA3 ura3-52 trp1 leu2-delta200 his3-delta200 pep4::HIS3 prbd1.6R can1 GAL

Preceptrol®

Comments

EBY100 (Leu-, Trp-) is BJ5465 and is MATa. It has auxotrophic: ura3-52 (a Ty element insertion with no detectable background reversion frequency), trp1 (an amber point mutation), leu2-delta200, his3-delta200, pep4HIS3, prbd1.6R, can1, GAL. EBY100 has genomic insertion of AGA1 regulated by GAL promoter with a URA3 selectable marker.

Morphology

Colonies on YEPD at 30°C after 4 days are butyrous, cream colored, smooth surface and usually flat. Cells are globose, ovoidal or elongate and are usually isolated or in small groups.

Medium

ATCC^® Medium 1245: YEPD
ATCC^® Medium 28: Emmons' modification of Sabouraud's agar
ATCC^® Medium 200: YM agar or YM broth

Growth Conditions

Temperature: 30°C

Atmosphere: Typical aerobic

Sequenced Data

18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence

GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTAAAGAAATTTAATAATTTTGAAAATGGATTTTTTTGTTTTGGCAAGAGCATGAGAGCTTTTACTGGGCAAGAAGACAAGAGATGGAGAGTCCAGCCGGGCCTGCGCTTAAGTGCGCGGTCTTGCTAGGCTTGTAAGTTTCTTTCTTGCTATTCCAAACGGTGAGAGATTTCTGTGCTTTTGTTATAGGACAATTAAAACCGTTTCAATACAACACACTGTGGAGTTTTCATATCTTTGCAACTTTTTCTTTGGGCATTCGAGCAATCGGGGCCCAGAGGTAACAAACACAAACAATTTTATCTATTCATTAAATTTTTGTCAAAAACAAGAATTTTCGTAACTGGAAATTTTAAAATATTAAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCCTTCTCAAACATTCTGTTTGGTAGTGAGTGATACTCTTTGGAGTTAACTTGAAATTGCTGGCCTTTTCATTGGATGTTTTTTTTCCAAAGAGAGGTTTCTCTGCGTGCTTGAGGTATAATGCAAGTACGGTCGTTTTAGGTTTTACCAACTGCGGCTAATCTTTTTTTATACTGAGCGTATTGGAACGTTATCGATAAGAAGAGAGCGTCTAGGCGAACAATGTTCTTAAAGTTTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTTAAGCATATCAATAA

D1D2 region of the 26S ribosomal RNA gene

ATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAGGGCATTTGATCAGACATGGTGTTTTGTGCCCTCTGCTCCTTGTGGGTAGGGGAATCTCGCATTTCACTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGCTTGCCTCGGTAAGTATTATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGTAAGTCAAGGATGCTGGCATAATGGTTATATGCCGC

Name of Depositor

KD Wittrup

Chain of Custody

ATCC <-- KD Wittrup

Isolation

Parent strain used Saccharoymes cerevisiae BJ5465.

Cross References

Nucleotide (GenBank) : KU729069 ITS including 5.8S rRNA gene

Nucleotide (GenBank) : KU729152 D1/D2 region of 26S rRNA gene

References

Boder ET, Wittrup KD. Yeast surface display for screening combinatorial polypeptide libraries. Nat Biotechnol 15: 553-557, 1997 PubMed: 9181578

Chen P, et al. Evaluation of immune protective efficacies of Eimeria tenella EtMic1 polypeptides with different domain recombination displayed on yeast surface. Exp. Parasitol. 155: 1-7, 2015. PubMed: 25956946

Zhou H, et al. Secretory expression and characterization of an acidic endo-polygalacturonase gene from Aspergillus niger SC323 in Saccharomyces cerevisiae. J. Microbiol. Biotechnol. 25: 999-1006, 2015. PubMed: 25737122

Geiser E, et al. Identification of an endo-1,4-beta-xylanase of Ustilago maydis. BMC Biotechnol. 13: 59, 2013. PubMed: 23889751

Blazic M, et al. Yeast surface display for the expression, purification and characterization of wild-type and B11 mutant glucose oxidases. Protein Expr Purif. 89: 175-180, 2013. PubMed: 23562736

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