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pTUM1.4

規(guī)格:

價格:

編號:B236994

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

pTUM1.4

商品貨號

B236994

Designations

pTUM1.4

Species

Turnip mosaic virus

培養(yǎng)基

Depositors

J Laliberte

Vector

Construct size (kb): 4.400000095367432

Insert

DNA: cDNA

Insert lengths(kb): 1.447000026702881

Gene product: capsid, 33 kDa

Insert Size (kb)

1.447

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--2.95, 0.9, 0.6; PstI--4.4; BamHI/HindIII--2.95, 1.45; XhoI--4.4; XbaI--3.0, 1.4.

The insert contains the following restriction sites, in kb from the 5' end: ClaI--0.156; EcoRI--0.906.

Isolated from naturally infected Brassica napus ssp. napobrassica (rutabaga) in the region of L'Assomption, Quebec, Canada.

Contains the complete capsid protein gene, part of the NIb gene, and part of the 3' end.

The 5' end of the insert begins 204 bp 5' to the published sequence and end at nt -506 of the published sequence.

Transcription from the T7 promoter gives the plus strand.

Classification

Potyvirus

References

Tremblay MF, et al. Sequence of the 3' terminal region of turnip mosaic virus RNA and the capsid protein gene. J. Gen. Virol. 71: 2769-2772, 1990. PubMed: 2254757

Jean-Francois Laliberte, personal communication

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