Codominant segregation of the BglII RFLP observed in 36 3-generation families.
PvuII and PstI also appear to reveal polymorphisms, but these have not been verified by pedigree studies.
RFLPs observed under normal conditions of hybridization and washing.
Vector
Construct size (kb): 4.699999809265137
Insert
DNA: genomic
Insert lengths(kb): 2.099999904632568
Gene product: DNA Segment, single copy [D10S13]
Alleles: A1, A2
Insert Size (kb)
2.100
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/BamHI--2.8, 1.9; EcoRI--2.8, 1.9; HindIII--4.2, 0.56.
Codominant segregation of the BglII RFLP observed in 36 3-generation families.
PvuII and PstI also appear to reveal polymorphisms, but these have not been verified by pedigree studies.
RFLPs observed under normal conditions of hybridization and washing.
Enzyme(s) not detecting polymorphism: EcoRI, BamHI, HindIII.
