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Monkey cytomegalovirus
Monkey cytomegalovirus
規格:
價格:
編號:B236761
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Monkey cytomegalovirus
商品貨號
B236761
Classification
Herpesviridae, Cytomegalovirus
Agent
Monkey cytomegalovirus
培養基
Strain
68-1
Common Name
African green monkey cytomegalovirus
Applications
Produces enlarged, distorted nuclei with bizarre inclusions infected human and monkey fibroblast TC. Has morphology of the herpes group (by EM).
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Urine of rhesus monkey, 1968
Product Format
frozen
Storage Conditions
-70°C or colder (or colder)
Comments
Sensitive to heat (exposure to 56C for 10 min. completely inactivated 10(4) TCID[50] of Rhesus CMV). Produces enlarged, distorted nuclei with bizarre inclusions infected human and monkey fibroblast TC. Has morphology of the herpes group (by EM).
Effect on Host
Yes, in vitro effects: Cytopathic effects (refractile rounding and sloughing)
CPE, refractile rounding and sloughing
Growth Medium
Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
Recommended Host

Recommended Host:? MRC-5 (ATCC CCL-171)

Growth Conditions
Atmosphere: 5% CO2 in air recommended
Temperature: 35.0°C
Duration: 3 to 30 days; For best results, cells should be 24 to 48 hours old and 70-80% confluent (not 100% confluent) at inoculation.
Cryopreservation
Storage temperature: -70°C
Name of Depositor
DM Asher
Source
Urine of rhesus monkey, 1968
Year of Origin
1968
References

Kaur A, et al. Cytotoxic T-lymphocyte responses to cytomegalovirus in normal and simian immunodeficiency virus-infected Rhesus macaques. J. Virol. 70: 7725-7733, 1996. PubMed: 8892893

Asher DM. . Bacteriol. Proc. 269: 91, 1969.

Asher DM, et al. Persistent shedding of cytomegalovirus in the urine of healthy Rhesus monkeys. Proc. Soc. Exp. Biol. Med. 145: 794-801, 1974. PubMed: 4131945

or colder

almost any human or monkey diploid fibroblast tissue culture cells may be used

or colder

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