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pMCR3
pMCR3
規(guī)格:
價(jià)格:
編號(hào):B236573
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pMCR3
商品貨號(hào)
B236573
Designations
pMCR3
Species
Homo sapiens, human
培養(yǎng)基
Depositors
RL White, Y Nakamura
Applications
The probe was pre-associated with total human DNA. Otherwise, RFLPs were observed under normal hybridization and wash stringencies
Vector
Construct size (kb): 12.0
Insert
DNA: genomic
Insert lengths(kb): 6.0
Gene product: neuroblastoma RAS viral (v-ras) oncogene homolog( v-ras neuroblastoma RAS viral oncogene homolog, proto-oncogene N-ras) [NRAS]
Alleles: A1, A2
Insert Size (kb)
6.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.65, 2.8, 2.8; HindIII--3.85, 3.85, 2.9, 0.86, 0.45; PstI--5.7, 3.1, 2.3, 1.6; BamHI--6.6, 3.0, 2.7, indicating two vectors + insert.
Codominant segregation demonstrated in 55 3-generation families for the EcoRI RFLP.
The probe was pre-associated with total human DNA. Otherwise, RFLPs were observed under normal hybridization and wash stringencies
Enzyme(s) not detecting polymorphism: MspI, TaqI, RsaI, BglII, PstI, PvuII, BamHI, HindIII.
References

Hall JM, et al. Oncogenes and human breast cancer. Am. J. Hum. Genet. 44: 577-584, 1989. PubMed: 2564734

Dracopoli NC, et al. The CEPH consortium linkage map of human chromosome 1. Genomics 9: 686-700, 1991. PubMed: 2037294

Carlson M, et al. Isolation and mapping of a polymorphic DNA sequence for NRAS pMCR3 on chromosome 1. Nucleic Acids Res. 15: 9623, 1987. PubMed: 2891121

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