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pUB110cop1

規格:

價格:

編號:B236565

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pUB110cop1

商品貨號

B236565

Designations

pUB110cop1

Depositors

H Leonhardt

培養基

Biosafety Level

Vector Information

Size (kb): 4.5000000000000000

Vector: pUB110cop1 (plasmid)

Promoters: Promoter none

Construction: pUB110, pEB114cop1

Marker(s):kanR,neoR

Construct size (kb): 4.5

Features: marker(s): kanR, neoR
promoter: none
replicon: pUB110

Applications

shuttle vector

vector with low copy number

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--4.5; NcoI--4.5; BamHI--4.5.

The copy number is 11 +/- 2 in Bacillus subtilis, resulting in an increase in structural and segregational stability.

Low copy number shuttle vector.

Constructed by replacing the NcoI/AflII fragment of pUB110 (nt 4033-4543) with the corresponding fragment from pEB114cop1 which contains a G -> T transversion in the Shine-Dalgarno sequence of repU at position 4130.

Media

ATCC Medium 1065 (see below) plus kanamycin/neomycin (3 mcg/ml) ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L

Growth Conditions

Temperature: 37.0°C

References

Gryczan TJ, et al. Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis. J. Bacteriol. 134: 318-329, 1978. PubMed: 418061

Gryczan T, et al. Characterization of chimeric plasmid cloning vehicles in Bacillus subtilis. J. Bacteriol. 141: 246-253, 1980. PubMed: 6243622

Leonhardt H. Identification of a low-copy-number mutation within the pUB110 replicon and its effect on plasmid stability in Bacillus subtilis. Gene 94: 121-124, 1990. PubMed: 2121616

Shipped

freeze-dried

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