最新中文字幕-精品国产99-国产精品福利在线观看-粉嫩av一区二区三区-日韩精品久久久久-美丽的姑娘国语视频-亚洲欧洲色-国产不卡毛片-三级黄毛片-免费福利在线-日韩av三区-欧美成年人网站-欧洲美女与动交ccoo-成人涩涩软件-秋霞影院午夜老牛影院-婷婷久久伊人-呦呦视频在线观看-www.天天综合-日韩精品高清视频-国产欧美综合在线-欧美日韩一区二区三区在线视频-a猛片免费播放-91九色蝌蚪国产-国产欧美日韩专区-嫩模被强到高潮呻吟不断

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > pAR1151 B236406
pAR1151
pAR1151
規格:
價格:
編號:B236406
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pAR1151
商品貨號
B236406
Designations
pAR1151
GenBank Number

J02518

培養基
Species
Escherichia coli bacteriophage T7
Depositors
Brookhaven National Laboratory, FW Studier, Brookhaven National Laboratory
Applications
produces protein RNA polymerase
Vector
Construct size (kb): 7.099999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: bacteriophage T7
Gene symbol: gene 1
Genomic copy number: unique
Gene name: RNA polymerase
Contains complete coding sequence?: U
Type of DNA: genomic
Insert end: Modification: BamHI linkers
Insert end: Modification: BamHI linkers
Insert size (kb): 2.696
Cross references: DNA Seq. Acc.: J02518
Insert lengths(kb): 2.696000099182129
Gene product: RNA polymerase [gene 1]
Target Gene: RNA polymerase
Insert Size (kb)
2.696
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.4, 2.8; BglII--uncut; EcoRI--7.2; PstI--7.2; HindIII--7.2.
The plasmid has no T7 promoter activity.
The insert includes 26 nucleotides of the natural gene 1 mRNA before the ATG initiation codon, 19 nucleotides from the natural mRNA beyond the gene 1 termination codon and has the natural ribosome-binding/translation initiation site.
The insert extends from nucleotides 3145 to 5841 of T7 DNA.
Gene 1 was sequenced from T7 wild-type DNA. The junctions between gene 1 and pBR322 in pAR1151 were sequenced.
This produces very low levels of T7 RNA polymerase, enough to complement a T7 gene 1 amber mutant.
References

Studier FW, et al. Cloning and expression of the gene for bacteriophage T7 RNA polymerase. US Patent 4,952,496 dated Aug 28 1990

Studier FW, Moffatt BA. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189: 113-130, 1986. PubMed: 3537305

Davanloo P, et al. Cloning and expression of the gene for bacteriophage T7 RNA polymerase. Proc. Natl. Acad. Sci. USA 81: 2035-2039, 1984. PubMed: 6371808

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
  • 王經理
    手機號：17280875617
    QQ：1438578920
  • 胡經理
    手機號：13345964880
    QQ：2438244627
  • 周經理
    手機號：17757487661
    QQ：1296385441
  • 于經理
    手機號：18067160830
    QQ：2088210172
  • 沈經理
    手機號：19548299266
    QQ：2662369050
  • 李經理
    手機號：13626845108
    QQ：972239479