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pHA68B12 [68B12, pHSA68B12]
pHA68B12 [68B12, pHSA68B12]
規(guī)格:
價格:
編號:B236237
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱
pHA68B12 [68B12, pHSA68B12]
商品貨號
B236237
Designations
pHA68B12 [68B12, pHSA68B12]
Species
Homo sapiens, human
培養(yǎng)基
Depositors
G Lucotte
Applications
THe second variant has been validated in family studies.
Vector
Construct size (kb): 5.300000190734863
Insert
DNA: cDNA
Insert lengths(kb): 1.0
Tissue: liver
Gene product: albumin [ALB]
Alleles: E1, C1, C2, D1, F1, F2, A1, D2, E2, A2
Insert Size (kb)
1.0
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Insert contains internal PstI (1), TaqI (2), MboII (2), HhaI (1), HinfI (2), HindIII (1), XbaI (1), and HincII (1) sites.
Restriction digests of the clone give the following sizes (kb): PstI--4.4, 0.9; EcoRI/NdeI--3.0, 2.3; EcoRI--5.3; HindIII--3.8, 1.5; BamHI--5.3.
The insert is from the 3' half of the cDNA.
No HaeIII is known in the cDNA of the albumin gene, so the variants must be intronic.
In one variant, 2 of 42 or f=0.05 were missing the 2.7 and 1.6 kb bands. In another variant, bands 4, 2.7, 0.7, 0.65, 0.6, and 0.55 were missing (f= 0.21). THe second variant has been validated in family studies.
When DNA is digested with HaeIII, the probe detects bands at 4, 3.4, 2.9, 2.7, 1.6, 0.95, 0.75, 0.72, 0.65, and 0.6 kb.
References

de Souza SL, et al. Pathologie moleculaire des genes albumine et alpha-foetoproteine. Hum. Genet. 67: 48-51, 1984. PubMed: 6086494

Mariotti M, et al. Individual polymorphism of albumin gene studied by sequencing data. Protides Biol. Fluids Proc. Colloq. 33: 177-179, 1985.

Lavareda de Souza S, et al. Polymorphisms of human albumin gene after restriction with HaeIII. Bio-sciences 5: 71-74, 1986.

Gerard Lucotte, personal communication

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