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pMMB278
pMMB278
規格:
價格:
編號:B236156
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pMMB278
商品貨號
B236156
Designations
pMMB278
Depositors
VM Morales
培養基
Biosafety Level
1
Vector Information
Size (kb): 8.9899997711181640
Vector: pMMB278 (plasmid)
Promoters: Promoter tac
Construction: pMMB190, pMMB311
Marker(s):ampR
Construct size (kb): 8.989999771118164
Features: insert detection: lacZ'
marker(s): ampR
promoter: tac
replicon: oriV, oriT (IncQ)
repressor gene: lacIq
transcription terminator: rrnB
Applications
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--9.0; EcoRV--5.4, 3.7; SspI--5.0, 1.7, 1.5, 0.9; EcoRI/MluI--8.2, 0.8.
Contains sequences encoding lacIq, a tac promoter, and lacZalpha to allow direct screening by inactivation of alpha-complementation.
Constructed by replacing the MluI/EcoRI fragment of pMMB190 (taclacUV5 promoter) with the MluI/EcoRI fragment of pMMB311 (tac promoter).
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Morales VM, et al. A series of wide-host-range low-copy-number vectors that allow direct screening for recombinants. Gene 97: 39-47, 1991. PubMed: 1847347

Shipped
freeze-dried
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