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pNMU-1

規(guī)格:

價(jià)格:

編號(hào):B235577

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

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甘油

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產(chǎn)品名稱

pNMU-1

商品貨號(hào)

B235577

Designations

pNMU-1

Species

Rattus norvegicus, rat

培養(yǎng)基

Depositors

M Barbacid

Vector

Construct size (kb): 11.39999961853027

Insert

DNA: genomic

Insert lengths(kb): 6.800000190734863

Tissue: transformed 3T3 cell line

Gene product: Harvey rat sarcoma viral (v-Ha-ras) oncogene homolog(Harvey rat sarcoma virus oncogene) [HRAS]

Insert Size (kb)

6.800

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Constructed from DNA from NIH 3T3 cell transformed by rat mammary tumor induced by nitroso-methyl urea.

Restriction digests of the clone give the following sizes (kb): BamHI--6.8, 4.5; HindIII--5.6, 3.15, 2.5; EcoRV--5.8, 5.2; EcoRI--11.5.

Insert contains NaeI, PvuII (3), SacI (3), HindIII (2), MstI, BstEII (2), HpaI (2), and EcoRV sites. Four exons demonstrated.

G to A transition in codon 12 leads to activation of this p21.

References

Sukumar S, et al. Induction of mammary carcinomas in rats by nitroso-methylurea involves malignant activation of H-ras-1 locus by single point mutations. Nature 306: 658-661, 1983. PubMed: 6318112

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