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PEmbl 600 [pEmbl 600 q]

規格:

價格:

編號:B235462

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

PEmbl 600 [pEmbl 600 q]

商品貨號

B235462

Designations

PEmbl 600 [pEmbl 600 q]

Species

Homo sapiens, human

培養基

Applications

DNA from secondary transfectants was used to isolate the clone.

Vector

Construct size (kb): 4.400000095367432

Insert

DNA: genomic

Insert lengths(kb): 0.6000000238418579

Tissue: NK14 cell line DNA transferred into NIH 3T3 cells

Gene product: mel transforming oncogene (derived from cell line NK14)- RAB8 homologue(ras-associated protein RAB8) [MEL]

Insert Size (kb)

0.600

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)

Comments

Restriction digests of the clone give the following sizes (kb): AvaI--3.85, 0.69; ClaI--4.4; EcoRI--4.4; SalI--4.4; HindIII--4.4.

Contains Sau3A (225), AccI (300), HinfI (345), DdeI (365), and SacII (540) sites (approximate distances in bp from 5' end). Suggest using the AvaI/Sau3A fragment as there are 50 bp of Alu sequences 3' to Sau3A site.

NIH 3T3 cells were transformed with DNA from the melanoma NK14 cell line. DNA from secondary transfectants was used to isolate the clone.

References

Padua RA, et al. A novel transforming gene in a human malignant melanoma cell line. Nature 311: 671-673, 1984. PubMed: 6090953

Spurr NK, et al. Chromosomal assignment of c-mel, a human transforming oncogene, to chromosome 19 (p13.2-q13.2). Somatic Cell Mol. Genet. 12: 637-640, 1986. PubMed: 3466361

Rose. Ann Padua, personal communication

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