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YpGX265GAL4 [GX4011]

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編號:B235443

品牌:Mingzhoubio

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產(chǎn)品名稱

YpGX265GAL4 [GX4011]

商品貨號

B235443

Designations

YpGX265GAL4 [GX4011]

Depositors

Genex Corp., SL Strausberg, Genex Corp.

培養(yǎng)基

U.S. Patent

5,013,652

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Biosafety Level

Vector Information

Size (kb): 12.3000001907348600

Vector: YpGX265GAL4 (plasmid)

Promoters: Promoter GAL1-10/MFalpha1

Construction: pJDB207, GAL4, control sequences: GAL1-10, MFalpha1, PHO5, GAPDH

Marker(s):ampR,LEU2-d

Construct size (kb): 12.30000019073486

Features: marker(s): ampR, LEU2-d
promoter: GAL1-10/MFalpha1
replicon: pMB1, 2 micron
terminator: GAPDH

Applications

YX-type (expression) shuttle vector

expression vector

shuttle vector

vector with sequences for protein export

Comments

Restriction digests of the clone give the following sizes (kb): EcoRV--5.1, 4.2, 3.0; BamHI--8.1, 4.2; HindIII--8.5, 3.8.

The plasmid is maintained at approximately 200 copies per cell in haploid S. cerevisiae cells.

LEU2-d is a promoter-defective allele of the yeast LEU2 gene, which increases plasmid copy number to 200-300 copies in the presence of LEU2 selection (host grown in medium lacking leucine).

High copy number YE-type shuttle expression vector.

Note that the XhoI, EcoRV and BamHI sites are not unique in this plasmid.

The promoter consists of nt -610 to -157 of the GAL 1-10 upstream activation site, fused to the transcription initiation site (nt -158 to -1) of the alpha mating factor gene MFalpha1.

The 3.65 kb HindIII fragment encodes the positive regulator protein GAL4, necessary for transcription from the GAL1-10 promoter.

The PHO5 signal sequence (51 nt) was modified to use preferred Saccharomyces codons.

The order of the major features in the plasmid is: HindIII - GAL4 - HindIII - Gal1-10 UAS - MFalpha1 transcription initiation sequence - PHO5 signal sequence - EcoRV - BamHI - GAPDH terminator - ampR - LEU2-d - 2 micron ori.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 39.0°C

References

Rose AB, Broach JR. Propagation and expression of cloned genes in yeast: 2-micron circle-based vectors. Methods Enzymol. 185: 234-279, 1990. PubMed: 2199781

Strausberg RL, Strausberg SL. Composite yeast vectors. US Patent 5,013,652 dated May 7 1991

Shipped

frozen

Shipping Information

Distributed: freeze-dried

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