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pGIKS-BioD
pGIKS-BioD
規(guī)格:
價格:
編號:B235360
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pGIKS-BioD
商品貨號
B235360
Designations
pGIKS-BioD
GenBank Number

J04423

5597)">J04423 (4947->5597)

培養(yǎng)基
Species
Escherichia coli (Migula) Castellani and Chalmers
Depositors
M Follettie
Applications
For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit).
Vector
Construct size (kb): 3.56
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II KS+
Intact vector size: 2.961
Type of vector: phagemid
Vector end: KpnI
Vector end: SacI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
replicon: pMB1, f1
Cross references:
Vector end (5'): KpnI
Vector end (3'): SacI
Excise insert: XhoI+SmaI
Promoter giving the sense strand: T3
Promoter giving the antisense strand: T7
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Escherichia coli
Gene symbol: bioD
Gene name: dethiobiotin synthetase
Contains complete coding sequence?: N
Type of DNA: genomic
Insert 5' end: XhoI Modification: KpnI linker
Insert 3' end: SmaI Modification: SacI linker
Insert size (kb): 0.66
Cross references: DNA Seq. Acc.: J04423
Nucleotides 1-651 of the insert correspond to
nucleotides 4947-5597 of J04423.
Insert lengths(kb): 0.66
Gene product: dethiobiotin synthetase [bioD]
Insert end (5'): KpnI linker
Insert end (3'): SacI linker
Insert Size (kb)
0.660
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit).
The insert contains nt 4947-5597 of Genbank J04423.
The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.18; PvuII--0.18; RsaI--0.64.
The following restriction enzymes are recommend for linearizing the plasmid for in vitro transcription: T3 = SmaI; T7 = XhoI.
Restriction digests of the clone give the following sizes (kb): SmaI--3.6; SmaI/XhoI--3.0, 0.6; XhoI--3.6.
Classification
Enterobacteriaceae, Escherichia
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