国产精品一区二区性色av,免费成人av在线,99精品在线免费观看

pNK2811

規格:

價格:

編號:B235259

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

加入購物車

在線咨詢收藏

產品名稱

pNK2811

商品貨號

B235259

Designations

pNK2811

Species

Escherichia coli (Migula) Castellani and Chalmers

培養基

Depositors

N Kleckner

Applications

produces protein transposase, Tn10

Vector

Construct size (kb): 9.199999809265137

Insert

DNA: genomic

Insert information:

Insert size (kb): 7.2

Gene : transposase, Tn10

DNA : genomic

Source : Escherichia coli

Sequenced ? Partial (see reference)

Insert lengths(kb): 7.199999809265137

Gene product: transposase, Tn10

Target Gene: transposase, Tn10

Insert Size (kb)

7.200

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Freeze dried E. coli containing the plasmid

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--9.2; HindIII--6.6, 2.5; PstI--5.2, 2.95, 0.98; XbaI--6.8, 2.1.

Construct permitting translational fusions of kan to target genes.

Kanamycin resistance is expressed after transpositions in the correct orientation and reading frame into an expressed target gene.

Contains a 2.2 kb mini-Tn10 cassette conferring erythromycin resistance and a promoterless kan gene ('kan) gene, bounded by inverted repeats of the outermost 70 bp of IS10R and embedded (as an EcoRI/HindIII fragment) in hisG and hisD.

Also contains the wild type transposase gene regulated by the Ptac promoter, inducible by IPTG, oriented opposite to 'kan, and separated from 'kan by 4 tandem repeats of the rrnB terminator.

The order of the major features in the insert is: XbaI - HindIII - transposase - Ptac - rrnB terminators - HindIII - 'kan - erm - EcoRI - XbaI.

Classification

Enterobacteriaceae, Escherichia

References

Kleckner N, et al. Uses of transposons with emphasis on Tn10. Methods Enzymol. 204: 139-180, 1991. PubMed: 1658561

王經理

手機號：17280875617

QQ：1438578920
胡經理

手機號：13345964880

QQ：2438244627
周經理

手機號：17757487661

QQ：1296385441
于經理

手機號：18067160830

QQ：2088210172
沈經理

手機號：19548299266

QQ：2662369050
李經理

手機號：13626845108

QQ：972239479