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phPMCA2-1 [lambdat10.1-3.0]

規格:

價格:

編號:B234814

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

phPMCA2-1 [lambdat10.1-3.0]

商品貨號

B234814

Designations

phPMCA2-1 [lambdat10.1-3.0]

GenBank Number

X63575

培養基

Species

Homo sapiens, human

Depositors

EE Strehler

Vector

Construct size (kb): 5.699999809265137

Insert

DNA: cDNA

Insert lengths(kb): 3.0

Tissue: teratoma NTera2D cell line

Gene product: ATPase, Ca++ transporting, plasma membrane 2 [PMCA2]

Alleles: A1, A2, A1, A2

Insert Size (kb)

3.0

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.7; PstI--2.8, 2.05, 0.55, 0.35; XhoI--5.7; BamHI--4.5, 1.1; HindIII--5.7.

The insert includes the following restriction sites (kb from the 5' end): PstI--0.21, 0.72, 2.68; XhoI--1.83; BamHI--1.86, 2.91; SacI--2.72, 2.82.

Extends from nt 410 to the EcoRI site at nt 3584, beginning at the codon for aa 114. Does not include 93 bp in the 5' coding region identified in some clones.

References

Heim R, et al. Microdiversity of human-plasma-membrane calcium-pump isoform 2 generated by alternative RNA splicing in the N-terminal coding region. Eur. J. Biochem. 205: 333-340, 1992. PubMed: 1313367

B236541：phPMCA2-1 [lambdat10.1-3.0]

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