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prbTRH479 [TPH479]
prbTRH479 [TPH479]
規格:
價格:
編號:B234412
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
prbTRH479 [TPH479]
商品貨號
B234412
Designations
prbTRH479 [TPH479]
GenBank Number

M17250

培養基
Species
Oryctolagus cuniculus, rabbit
Depositors
SL Woo
Vector
Construct size (kb): 11.14000034332275
Insert
DNA: cDNA
Insert lengths(kb): 2.440999984741211
Tissue: pineal body
Gene product: tryptophan hydroxylase (tryptophan 5-monooxygenase) [TPH]
Insert Size (kb)
2.441
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
The following restriction enzymes do not cut the insert: AatII, BalI, BamHI, BglI, BglII, BstEII, BstXI, HpaI, KpnI, MluI, MstI, NaeI, NarI, NcoI, NdeI, NruI, PaeR7I, PstI, PvuI, SalI, SfiI, SmaI, XbaI, and XhoI.
Restriction sites (with nucleotide positions relative to the published sequence) include: AvaII--476; BclI--1548; EcoRI--624; EcoRV--747; HincII--323; HindIII--1262; PvuII--770, 1454; SacI--673, 1833; XmnI--240.
Restriction digests of the clone give the following sizes (kb): EcoRI--8.6, 1.9, 0.6; HindIII--6.3, 1.7, 1.3, 1.2, 0.5; BamHI--6.5, 3.2, 1.0; consistent with deposited DNA.
The insert contains an EcoRI site at nucleotide 624 so that an EcoRI digest yields 2 fragments from the insert.
Contains the complete coding sequence (nt 132-1464).
References

Grenett HE, et al. Full-length cDNA for rabbit tryptophan hydroxylase: functional domains and evolution of aromatic amino acid hydroxylases. Proc. Natl. Acad. Sci. USA 84: 5530-5534, 1987. PubMed: 3475690

Savio L Woo, personal communication

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