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pLRbRNL
pLRbRNL
規格:
價格:
編號:B234266
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pLRbRNL
商品貨號
B234266
Designations
pLRbRNL
GenBank Number

M28419

培養基
Species
Homo sapiens, human
Depositors
W Lee
Applications
in another host, produces protein retinoblastoma 1 (including osteosarcoma)
Vector
Construct size (kb): 10.39999961853027
Insert
DNA: cDNA
Insert lengths(kb): 3.700000047683716
Gene product: retinoblastoma 1 (including osteosarcoma) [RB1]
Alleles: A1, A2, A1, A2
Target Gene: retinoblastoma 1 (including osteosarcoma)
Insert Size (kb)
3.700
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
The insert contains an EcoRI site (1028) and a BglII site (1970).
Restriction digests of the clone give the following sizes (kb): HindIII/ClaI--4.3 (doublet), 1.8; EcoRI--4.5, 3.5, 1.1, 0.9; HindIII--6.0, 4.3; BamHI--6.5, 4.0; PstI--3.8, 2.8, 2.0, 1.9.
The O.9 kb EcoRI/BglII fragment detects genomic HindIII fragments of the following sizes (kb): 7.0, 1.3, 5.5, 4.5, 7.5, 5.3.
The insert consists of the first 3686 nucleotides of the published sequence, including the complete coding sequence (138 - 2922) plus 5' untranslated and partial 3' untranslated regions.
The construct expresses a normal-sized protein product in transfected mouse and rat cells.
This plasmid was constructed from a 4.3 kb HindIII/ClaI fragment containing 2 MoMLV LTR sequences flanking a beta-lactamase gene, a 2.4 kb SmaI/ClaI fragment containing RSV-promoter-Neo, and the 3.7 kb HindIII/ScaI cDNA fragment.
This sequence in the insert was constructed from cDNAs cloned from several sources.
References

Huang HJ, et al. Suppression of the neoplastic phenotype by replacement of the RB gene in human cancer cells. Science 242: 1563-1566, 1988. PubMed: 3201247

Lee WH, et al. Human retinoblastoma susceptibility gene: cloning, identification, and sequence. Science 235: 1394-1399, 1987. PubMed: 3823889

Lee WH, et al. The retinoblastoma susceptibility gene encodes a nuclear phosphoprotein associated with DNA binding activity. Nature 329: 642-645, 1987. PubMed: 3657987

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