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49a
49a
規(guī)格:
價格:
編號:B234220
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
49a
商品貨號
B234220
Designations
49a
GenBank Number

S86117

培養(yǎng)基
Species
Homo sapiens, human
Depositors
J Weissenbach
Vector
Construct size (kb): 12.5
Insert
DNA: genomic
Insert lengths(kb): 7.099999904632568
Gene product: DNA Segment, single copy [DYS1]
Alleles: B2, D4, A5, B1, C2, D3, D4, F2, I2, D2, A1, A2, A3, A4, C1, D1, F1, I1
Insert Size (kb)
7.100
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
This plasmid was constructed by linkering XbaI/Sau3A fragments from the Y chromosome with EcoRI and inserting into the EcoRI site of pJB8. There are EcoRI sites in the insert so the intact insert cannot be released by an EcoRI digest.
Restriction digests of the clone give the following sizes (kb): BamHI--11.0; BamHI/XbaI--10.0, 0.94; HindIII--6.4, 4.7; SalI--12.5; EcoRI--5.4, 4.2, 0.66, 0.52.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
The single-copy BamHI/XbaI fragment has been sequenced.
The single-copy probe (0.9 kb) of the insert can be excised with a BamHI/XbaI double digest. It must be separated from highly repeated flanking sequence by gel electrophoresis.
At low stringency (2X SSC, 42C) TaqI detects 4 Y-specific bands which are independently polymorphic and several other Y-specific constant bands.
References

Ngo KY, et al. A DNA probe detecting multiple haplotypes of the human Y chromosome. Am. J. Hum. Genet. 38: 407-418, 1986. PubMed: 3010708

Spurdle A, Jenkins T. Y chromosome probe p49a detects complex PvuII haplotypes and many new TaqI haplotypes in southern African populations. Am. J. Hum. Genet. 50: 107-125, 1992. PubMed: 1729883

Lucotte G, et al. Nucleotide sequence of p49a, a genomic Y-specific probe with potential utilization in sex determination. Mol. Cell. Probes 5: 359-363, 1991. PubMed: 1791857

Jean Weissenbach, personal communication

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