Vector Information
Size (kb): 7.3769998550415040
DESCRIPTION OF VECTOR:
Intact vector size: 7.377
Type of vector: plasmid
Cloning sites: NotI SacI SmaI BamHI XbaI
Polylinker sites: NotI EcoRI SacI AvaI KpnI SmaI BamHI XbaI
Other unique sites: HindIII
Host range: Saccharomyces cerevisiae; Escherichia coli;
Schizosaccharomyces pombe
Features (with orientation and position when available):
restriction site: HindIII, 1-1
promoter for in vitro transcription: T3, <-, 11-30
promoter: lac, <-, 100-128
replicon: pMB1, 454-454
marker(s): ampR, <-, 1212-2072
promoter for in vitro transcription: T7, ->, 3132-3151
MCS: NotI...XbaI, ->, 3180-3214
replicon: 2 micron/LEU2-d, 3208-5903
marker(s): LEU2-d, ->, 4570-5664
marker(s): URA4 from S. pombe, <-, 6055-6849
Vector: YEpFAT2 (plasmid)
Promoters: Promoter for in vitro transcription T3
Marker(s):ampR,LEU2-d,ura4+
Construct size (kb): 7.376999855041504
Features: marker(s): LEU2-d
marker(s): URA4 from S. pombe
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: 2 micron/LEU2-d
replicon: pMB1
MCS: NotI...XbaI
restriction site: HindIII
