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pDSV100

規格:

價格:

編號:B230921

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pDSV100

商品貨號

B230921

Designations

pDSV100

GenBank Number

M23022

培養基

Species

Digitaria streak virus

Depositors

PM Mullineaux, GP Accotto

Vector

Construct size (kb): 3.98799991607666

Insert

DNA: cDNA

Insert lengths(kb): 0.8799999952316284

Gene product: coat protein V2

Insert Size (kb)

0.880

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Restriction digests of the clone give the following sizes (kb): SacI--4.0; HindIII--4.0; SalI--4.0; PstI--3.3, 0.7.

The insert contains the following restriction sites (nt position according the published sequence): KpnI--249, 674, 1008; PstI--454; SacI--506; NruI--883.

Linearizing with SphI or HindIII and transcription with SP6 RNA polymerase will give the sense strand.

The SP6-derived transcript would direct the translation of the V2 (coat protein) ORF in an in vitro translation system, such as wheat germ or rabbit reticulocyte lysate.

Constructed from the MluI/SpeI fragment extending from nt 183 to 1071 of the published sequence. The EcoRI/MluI and SpeI/XbaI sites were destroyed.

Classification

Geminivirus

References

Donson J, et al. The nucleotide sequence of a geminivirus from Digitaria sanguinalis. Virology 161: 160-169, 1987. PubMed: 3672928

P M Mullineaux, personal communication

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