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pTC4.5

規格:

價格:

編號:B229496

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pTC4.5

商品貨號

B229496

Designations

pTC4.5

GenBank Number

L22484

培養基

Species

Trypanosoma cruzi Chagas

Depositors

LM Ruiz-Perez

Vector

Construct size (kb): 7.190000057220459

Insert

DNA: genomic

Insert lengths(kb): 4.5

Gene product: dihydrofolate reductase-thymidylate synthase [DHFR-TS]

Insert Size (kb)

4.5

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI/PstI--4.6, 2.8; HindIII--7.4; SalI--7.4.

The insert contains the following restriction sites (approximate from the 5' end): BstXI--3.95; NcoI--2.52; SalI--2.48; SphI--2.42, 2.76, 3.65; XhoI--2.79.

The insert contains the following components (listed 5'->3'): a PstI/SalI fragment from the MCS of lambda EMBL3, 2.4 kb upstream of the coding sequence for DHFR-TS, the 1.56 kb coding region, and 0.44 kb downstream of DHFR-TS.

All but 100 bp of the coding sequence is in a 1.9 kb SalI/EcoRI fragment.

Classification

KINGDOM: Protozoa

References

Reche P, et al. Cloning and expression of the dihydrofolate reductase-thymidylate synthase gene from Trypanosoma cruzi. Mol. Biochem. Parasitol. 65: 247-258, 1994. PubMed: 7969266

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