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pBlueBac/PKCdelta
pBlueBac/PKCdelta
規格:
價格:
編號:B228558
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pBlueBac/PKCdelta
商品貨號
B228558
Designations
pBlueBac/PKCdelta
GenBank Number

L07860

培養基
Species
Homo sapiens, human
Applications
in another host, produces protein protein kinase C, delta
Vector
Construct size (kb): 16.10000038146973
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBlueBac
Intact vector size: 14.000
Type of vector: phagemid
Vector end: NheI
Vector end: NheI
Cloning sites: NheI
Polylinker sites:
Host range: insect cells; Escherichia coli
Features (with orientation and position when available):
insert detection: lac
marker(s): ampR
promoter: polyhedrin
replicon: pMB1, f1, SV40
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: PRKCD
Gene name: protein kinase C, delta
Contains complete coding sequence?: Y
Localization: 3
Tissue: liver, HepG2 cell line
Type of DNA: cDNA
Insert end: Modification: NheI linkers
Insert end: Modification: NheI linkers
Insert size (kb): 2.1
Cross references: DNA Seq. Acc.: L07860
Insert lengths(kb): 2.099999904632568
Tissue: liver, HepG2 cell line
Gene product: protein kinase C, delta [PRKCD]
Target Gene: protein kinase C, delta
Insert Size (kb)
2.100
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): NheI--14.0, 2.1; BglI--5.6, 5.4, 2.2, 1.8, 1.2; HindIII--8.8, 4.0, 1.3, 1.1, 1.0; NcoI--14.0, 1.25, 0.5, 0.35; PstI--14.0, 1.1, 0.55, 0.3.
Insert contains the following restriction sites (approximate kb from the 5' end): BglI--1.13; BglII--1.01; HindIII--0.85; NcoI--1.18, 1.64, 1.96; PstI--0.05, 0.33, 1.39; SalI--0.17.
Contains the complete coding sequence, and begins at the initiation codon (nt 59 of the sequence record).
References

Aris JP, et al. Molecular and biochemical characterization of a recombinant human PKC-delta family member. Biochim. Biophys. Acta 1174: 171-181, 1993. PubMed: 8357834

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