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pEA974
pEA974
規(guī)格:
價(jià)格:
編號(hào):B226858
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pEA974
商品貨號(hào)
B226858
Designations
pEA974
Species
Cricetus cricetus, hamster
培養(yǎng)基
Depositors
N Robakis
Vector
Construct size (kb): 5.400000095367432
Insert
DNA: cDNA
Insert lengths(kb): 0.9739999771118164
Tissue: scrapie-infected brain
Gene product: prion protein (p27-30)(prion protein) [PRIP]
Alleles: A1, A1, A1, A1, A2, A2, B1, B1, B2, B2, A2, A2
Insert Size (kb)
0.974
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--4.4, 0.9; EcoRI--5.4; HindIII--5.4; BamHI--5.4; PstI--5.4.
Insert includes sequence from nt 2 to nt 808, with first in-frame ATG at nt 47, encoding a protein of 254 amino acids. Hybridization conditions suggest homology between hamster and human sequences is about 80%.
Hybridizes to a unique human genomic BamHI fragment of 10 kb (0.1X SSC, 68C) and human mRNA of about 3 kb.
Enzyme(s) not detecting polymorphism: ApaI, ApaLI, AvaI, AvuI, BanI, BamHI, BclI, BglI, BglII, BstEII, BstNI, EcoRI, EcoRV, HaeIII, HindIII, HinfI, KpnI, MspI, NheI, NruI, PstI, RsaI, SmaI, SstI, StuI, TaqI, XbaI, XmnI (20 unrelated European
References

Harris MS, et al. A BanI RFLP at a deletion hotspot in the human dystrophin gene. Nucleic Acids Res. 18: 385, 1990. PubMed: 1970164

Wu Y, et al. A PvuII RFLP detected in the human prion protein (PrP) gene. Nucleic Acids Res. 15: 3191, 1987. PubMed: 2882483

Robakis NK, et al. Localization of a human gene homologous to the PrP gene on the p arm of chromosome 20 and detection of PrP-related antigens in normal human brain. Biochem. Biophys. Res. Commun. 140: 758-765, 1986. PubMed: 2877664

Robakis NK, et al. Isolation of a cDNA clone encoding the leader peptide of prion protein and expression of the homologous gene in various tissues. Proc. Natl. Acad. Sci. USA 83: 6377-6381, 1986. PubMed: 3529083

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