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Rickettsia raoultii

規格:

貨期:

編號:B225239

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

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產品名稱

Rickettsia raoultii

商品貨號

B225239

Classification

Rickettsiaceae, Rickettsieae

Agent

Rickettsia raoultii

Strain

DnS14

Applications

Vector borne research

Positive for Mycoplasma

Yes

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation

Isolated from Dermacentor nutalli in Siberia, in 1994

Product Format

frozen

Storage Conditions

?Store at -60°C or colder

Comments

This item is a representative of a newly identified rickettsial species (Rickettsia raoultii), a gram-negative, obligately intracellular and non-motile bacterium.

It is genetically identical to the type strain for this species (Khabarovsk, isolated in 2005) at all loci sequenced. The preparation available through ATCC tested positive for mycoplasma contamination (M.orale) by PCR.

Requests for this agent require special permits and a signed statement assuming all risks and responsibility for laboratory handling.

Bacteria visible in cells following staining with Gimenez stain

Effect on Host

CPE in the form of cell rounding and sloughing of infected cells.

Growth Medium

ATCC^® Medium 30-2003: Eagle's Minimum Essential Medium (EMEM)
Fetal Bovine Serum

Recommended Host

Vero cells (ATCC CRL-1587)

Growth Conditions

Recommendation for Infection: 5 days at 32°C and 5% CO₂ in EMEM (ATCC 30-2003; with 2 mM L-glutamine) supplememted with 2% FBS (ATCC 30-2020), following centrifugation at 700 g for one hour at 22°C

Name of Depositor

Dr. P. -E. Fournier

Special Collection

ATCC

Year of Origin

1994

References

Mediannikov, O., et. al. 2008. Rickettsia raoultii sp. nov., a spotted fever group rickettsia associated with Dermacentor ticks in Europe and Russia. Int. J Syst. Evol. Microbiol. Jul;58 (Pt 7):1635-39. PubMed: 18599708

Marrero, M and Raoult D. Centrifugation-shell vial technique for rapid detection of Mediterranean spotted fever rickettsia in blood culture. Am J Trop Med Hyg. 1989 Feb;40(2):197-9. PubMed: 2645804

Rydkina, E. et. al., New Rickettsiae in ticks collected in territories of the former soviet union. Emerg. Infect. Dis. 1999. Nov-Dec;5(6):811-4. PubMed: 10603217

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