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pBSVH167

規格:

價格:

編號:B221800

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pBSVH167

商品貨號

B221800

Designations

pBSVH167

Species

Mus musculus, mouse

培養基

Depositors

U Storb

Vector

Construct size (kb): 2.700000047683716

Insert

DNA: genomic

Insert lengths(kb): 0.1889999955892563

Tissue: myeloma MOPC-167 cell line

Gene product: immunoglobulin heavy-chain variable region [IghV]

Insert Size (kb)

0.189

Media

ATCC^® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.4, 0.23; SmaI--uncut; EcoRI--2.7; BamHI--2.7; HindIII--2.7; EcoRI/BamHI--2.5, 0.2.

The antibodies encoded by myeloma MOPC-167 are specific to phosphorylcholine.

The insert was subcloned from ChM167alpha10.1. A 210 bp fragment can be excised by an EcoRI/BamHI double digest, which cuts in the polylinker.

The vector pBSc was constructed by inserting the 382 bp PvuII fragment from pBluescribe (Stratagene) into pACYC184 (pUC8CmR) which had been partially digested with PvuII.

Classification

Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod

References

Storb U, et al. Trangenic mice with mu and kappa genes encoding antiphosphorylcholine antibodies. J. Exp. Med. 164: 627-641, 1986. PubMed: 2425036

Ursula Storb, personal communication

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