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pC194Amy
pC194Amy
規格:
價格:
編號:B220976
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pC194Amy
商品貨號
B220976
Designations
pC194Amy
Species
Geobacillus stearothermophilus (Donk) Nazina et al.
培養基
Depositors
CPC International, Inc.
Applications
produces protein amylase, alpha 1A; salivary Amylase, salivary, alpha-1A
Vector
Construct size (kb): 8.300000190734863
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pC194
Intact vector size: 2.900
Type of vector: plasmid
Vector end: HindIII
Vector end: HindIII
Cloning sites: HaeIII HindIII BglI
Polylinker sites:
Construction: natural isolate
Host range: Bacillus sp.; Staphylococcus aureus
Features (with orientation and position when available):
marker(s): cmlR
replicon: pC194
Cross references: DNA Seq. Acc.: L08860
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pC194
Intact vector size: 2.900
Type of vector: plasmid
Vector end: HindIII
Vector end: HindIII
Cloning sites: HaeIII HindIII BglI
Polylinker sites:
Construction: natural isolate
Host range: Bacillus sp.; Staphylococcus aureus
Features (with orientation and position when available):
marker(s): cmlR
replicon: pC194
Cross references: DNA Seq. Acc.: L08860
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Bacillus stearothermophilus
Gene name: alpha-amylase
Contains complete coding sequence?: U
Strain: N21
Type of DNA: genomic
Insert end: HindIII
Insert end: HindIII
Insert size (kb): 3.0
Cross references:
DESCRIPTION OF INSERT COMPONENT:
Genome: Bacillus stearothermophilus
Gene name: alpha-amylase
Contains complete coding sequence?: U
Strain: N21
Type of DNA: genomic
Insert end: HindIII
Insert end: HindIII
Insert size (kb): 3.0
Cross references:
Insert lengths(kb): 3.0
Gene product: amylase, alpha 1A; salivary(Amylase, salivary, alpha-1A)
Target Gene: amylase, alpha 1A; salivary
Insert Size (kb)
3.0
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Plasmid encodes a thermostable alpha-amylase which is exported.
The probe was constructed from pC194 and Bacillus stearothermophilus plasmid DNA.
References

Mielenz JR, Mickel S. Process for cloning the gene coding for a thermostable alpha-amylase into Escherichia coli and Bacillus subtilis. US Patent 4,493,893 dated Jan 15 1985

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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