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5B1-4

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編號:B220841

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

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產品名稱

5B1-4

商品貨號

B220841

Designations

5B1-4

GenBank Number

L10338

培養基

Species

Homo sapiens, human

Depositors

JF Gusella

Vector

Construct size (kb): 4.400000095367432

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites:
Polylinker sites:
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SCN1B
Gene name: sodium channel, voltage-gated, type I, beta polypeptide
Contains complete coding sequence?: Y
Chromosome: 19; Localization: 19 q13.1
Tissue: frontal cortex
Type of DNA: cDNA
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 1.404
Cross references: DNA Seq. Acc.: L10338
Nucleotides 1-1404 of the insert correspond to
nucleotides 1-1404 of L10338 with 100% identity.

Insert lengths(kb): 1.404000043869019

Tissue: frontal cortex

Gene product: sodium channel, voltage-gated, type I, beta polypeptide(sodium channel, voltage-gated, beta 1 polypeptide) [SCN1B]

Insert Size (kb)

1.404

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): BglI--1.7, 1.3, 0.72 (doublet); EcoRI--2.9, 1.5; PstI--3.9, 0.45; PvuII--2.5, 1.25, 0.62; SstI--4.4.

The insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.28, 0.95; NdeI--0.61; NcoI--0.10; PstI--0.40; PvuII--0.33; SmaI--0.06.

Detects a 1.5 kb transcript in several brain regions and skeletal muscle.

References

McClatchey AI, et al. The cloning and expression of a sodium channel beta 1-subunit cDNA from human brain. Hum. Mol. Genet. 2: 745-749, 1993. PubMed: 8394762

B237850：5B1-4

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