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BLUR8 [pPD8]
BLUR8 [pPD8]
規格:
價格:
編號:B217067
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
BLUR8 [pPD8]
商品貨號
B217067
Designations
BLUR8 [pPD8]
GenBank Number

J00091

培養基
Species
Homo sapiens, human
Depositors
PL Deininger
Vector
Construct size (kb): 4.639999866485596
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Genomic copy number: repetitive
Gene name: Alu consensus sequence, repetitive
Contains complete coding sequence?: U
Type of DNA: genomic
Insert end: Modification: BamHI linkers
Insert end: Modification: BamHI linkers
Insert size (kb): 0.275
Cross references: DNA Seq. Acc.: J00091
Insert lengths(kb): 0.2750000059604645
Gene product: Alu consensus sequence, repetitive
Insert Size (kb)
0.275
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
EcoRI--4.7; HindIII--4.7; BamHI--4.4, 0.27; PvuII--4.7; PstI--4.7.
Because of the BamHI linkers (CCGGATCCGG), the size of the excised fragment is 275 bp, with 265 bp representing the Alu repeat.
Consists of a head to tail dimer of approximately 130 bp with an insert of 31 bp in the right half as compared to the left. There is a 22% mismatch between BLUR8 and the average Alu family sequence (cf. GenBank accession J00092).
References

Rubin CM, et al. Partial nucleotide sequence of the 300-nucleotide interspersed repeated human DNA sequences. Nature 284: 372-374, 1980. PubMed: 6244506

Deininger PL, et al. Base sequence studies of 300 nucleotide renatured repeated human DNA clones. J. Mol. Biol. 151: 17-33, 1981. PubMed: 6276559

Prescott L Deininger, personal communication

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