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The medium contains a stable glutamine dipeptide, providing  a long shelf-life when handled and stored properly medium may be supplemented by addition of ES-Cell  Qualified Fetal Bovine Serum (FBS) or a serum replacement  designed for mouse ES cells. This medium can support the growth of mouse ES cells on  either a mitotically inactivated layer of mouse embryonic  fibroblast (MEF) feeder cells, or in a feeder-free environment using gelatin-coated plates.

Sterility tests are performed on each lot of medium using current USP methods. Each lot of medium is also checked for its ability to support the growth of several different cell lines using both sequential subcultures and plating efficiency. Additional test results are listed in the Certificate of Analysis.