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Canine parvovirus

規(guī)格:

貨期:

編號(hào):B212795

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

Canine parvovirus

商品貨號(hào)

B212795

Classification

Parvoviridae, Parvovirus, Feline panleukopenia virus

Agent

Canine parvovirus

Strain

Cornell-780916-80

Applications

Efficacy testing

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format

freeze-dried

Storage Conditions

?-70°C or colder?

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Comments

Reduced virulence variant obtained by passage at 33°C.

For best titer and CPE, infect actively growing cells. Virus agglutinates pig and rhesus monkey erythrocytes.

Effect on Host

CPE; refractile cell rounding and sloughing

Recommended Host

A-72 (ATCC^® CRL-1542™)

Growth Conditions

Temperature: 37°C
Incubation: Incubate infected A-72 cell culture for 3-5 days at 37°C in 100% atmospheric air, until CPE is well advanced through 90% of the culture.
Recommendations for Infection: Plant cells 6 hours in advance and infect when cultures are 40-50% confluent. Remove medium and inoculate with a small volume (e.g. 1 mL per 25 cm² ) of virus diluted with virus growth medium to provide a MOI of about 1.0. Adsorb 1 hour at 37°C in 100% atmospheric air in nonvented flasks. End adsorption by adding viral growth media.

Name of Depositor

Cornell Res. Fndn., Inc.

Source

Intestinal contents of beagle pups with severe enteritis

References

Carmichael LE, et al. Hemagglutination by canine parvovirus: serologic studies and diagnostic applications. Am. J. Vet. Res. 40: 784-791, 1980. PubMed: 6250432

Standard Quantitative Disk Carrier Test Method for Determining the Bactericidal, Virucidal, Fungicidal, Mycobactericidal and Sporicidal Activities of Liquid Chemical Germicides. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2197-02.

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