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pGB29

規格:

價格:

編號:B211048

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pGB29

商品貨號

B211048

Designations

pGB29

GenBank Number

J05411

培養基

Species

Elizabethkingia miricola

Depositors

Genzyme Corp., G Barsomian, Genzyme Corp.

Applications

produces protein peptide-N(4)-(N-acetyl-beta-D-glucosaminyl) asparagine amidase F

Vector

Construct size (kb): 6.200

Insert

DNA: genomic

Insert lengths(kb): 3.30

Gene product: peptide-N(4)-(N-acetyl-beta-D-glucosaminyl) asparagine amidase F [ngl]

Target Gene: peptide-N(4)-(N-acetyl-beta-D-glucosaminyl) asparagine amidase F

Insert Size (kb)

3.300

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Shipped: ?Freeze dried E. coli containing the phagemid.

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--uncut; Sau3AI--1.0, 0.7, 0.6, + smaller; BglII--3.5, 3.2.

The sequenced portion of the insert contains the following restriction sites (approximate kb from the 5' end): EcoRV--1.33; HindIII--0.80; NdeI--0.85; PvuII--0.93; EcoRI--1.6. Additional EcoRI sites are known to occur beyond the site indicated.

Contains the complete coding sequence (nt 139-1203). A subclone deleting EcoRI fragments downstream of the gene was used for sequencing and expression studies.

Proteins of 35, 37 and 38 kDa are expressed. Expression is independent of orientation in the vector, suggesting the presence of an endogenous promoter that functions in E. coli.

The vector was constructed from pBluescript SK+ by converting ApaI and SacI sites into BglII sites.

The insert in this clone was isolated from ATCC 33958, Elizabethkingia miricola, formerly Flavobacterium meningosepticum.

References

Barsomian GD, et al. Cloning and expression of peptide-N(4)-(N-acetyl-beta-D-glucosaminyl) asparagine amidase F in Escherichia coli. J. Biol. Chem. 265: 6967-6972, 1990. PubMed: 2182635

Barsomian GD, et al. Endo F-free PNGase. US Patent 5,238,821 dated Aug 24 1993

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

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