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hPKCalpha-GF29

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價格:

編號:B210421

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

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產品名稱

hPKCalpha-GF29

商品貨號

B210421

Designations

hPKCalpha-GF29

GenBank Number

X52479

培養基

Species

Homo sapiens, human

Depositors

H Hug

Vector

Construct size (kb): 5.199999809265137

Insert

DNA: cDNA

Insert lengths(kb): 2.244999885559082

Tissue: acute T cell leukemia, Jurkat cell line

Gene product: protein kinase C, alpha [PKCA]

Insert Size (kb)

2.245

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.3; BamHI--4.5, 0.65, 0.25; BglI--2.7, 1.3 (doublet); NcoI--4.2, 1.2; PvuII--2.4, 1.4, 0.75, 0.6.

The insert contains the following restriction sites (bp from the 5' end): BamHI--585, 796; BglI--965; NcoI--26, 1178; PstI--178; PvuII--1233, 1951; SmaI--44.

Contains the complete coding sequence (nt 28-2046).

The insert is oriented such that transcription from T7 gives the sense strand and from T3 gives the antisense strand.

References

Finkenzeller G, et al. Sequence of human protein kinase C alpha. Nucleic Acids Res. 18: 2183, 1990. PubMed: 2336401

Hubert Hug, personal communication

B172735：hPKCalpha-GF29

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