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pIP63
pIP63
規格:
價格:
編號:B210284
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pIP63
商品貨號
B210284
Designations
pIP63
GenBank Number

L12376

培養基
Species
Drosophila melanogaster, fruit fly
Depositors
WJ Van De Ven
Applications
produces protein Dfurin 1
Vector
Construct size (kb): 6.800000190734863
Insert
DNA: cDNA
Insert lengths(kb): 3.799999952316284
Tissue: embryo
Gene product: Dfurin 1 [Dfur1]
Target Gene: Dfurin 1
Insert Size (kb)
3.800
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.8, 3.0; NotI--3.8, 3.0; BamHI--5.5, 1.3; PstI--4.3, 2.5; PvuII--2.5, 2.2, 1.05, 0.6, 0.45.
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--1.12; PstI--2.43, 2.46; SalI--2.65; PvuII--2.51, 2.92.
Encodes the isoform produced from 4.0 and 6.5 kb transcripts.
Contains the complete coding sequence (nt 627-3305). Corresponds exactly to GenBank accession L12376.
References

Van de Ven WJ, et al. Furin: the prototype mammalian subtilisin-like proprotein-processing enzyme. Endoproteolytic cleavage at paired basic residues of proproteins of the eukaryotic secretory pathway. Enzyme 45: 257-270, 1991. PubMed: 1843280

Roebroek AJ, et al. Generation of structural and functional diversity in furin-like proteins in Drosophila melanogaster by alternative splicing of the Dfur1 gene. EMBO J. 12: 1853-1870, 1993. PubMed: 8491178

Wim J Van De Ven, personal communication

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