久久久国产精,男人在线网站,日韩视频在线一区

pSW253

規格:

價格:

編號:B206341

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

加入購物車

在線咨詢收藏

產品名稱

pSW253

商品貨號

B206341

Designations

pSW253

GenBank Definition

cloning vector pBR322, complete genome

培養基

GenBank Number

K02537

J01749

Species

reticuloendotheliosis virus-A

Depositors

TJ Jacoby

Vector

Construct size (kb): 19.70

Insert

DNA: genomic

Insert lengths(kb): 15.3

Insert Size (kb)

15.300

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Shipped: ?Frozen glycerol stock of E. coli containing the plasmid

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--15.3, 4.3; HindIII--7.3, 6.0, 4.1, 1.3, 1.0; BamHI--9.7, 6.0, 2.2, 1.8.

The insert contains the following restriction sites (approximate kb from one end): HindIII--0.3 (spleen DNA), 7.8, 9.3 (viral); KpnI--2.7, 3.6, 5.6 (viral), 14.5 (spleen); SalI--3.0 (viral); XhoI--6.7 (viral); SacI--2.0, 2.8, 9.9 (viral), 11.3, 13.2.

Size of construct: 19.7kb.

Full-length clone of reticuloendotheliosis virus-A (pSW253) inserted at EcoRI site in pBR322; construct is amp resistant and tet resistant. See also GenBank/EMBL/DDBJ J01749.

Corresponds to the larger EcoRI fragment of clone 2-20-6. GenBank K02357 begins 170 bp upstream of the XhoI site.

The insert contains the 8.3 kb REV-A proviral DNA and flanking chicken spleen sequences subcloned from clone 2-20-6. The proviral sequences begin about 2 kb from the EcoRI site closer to the HindIII site.

References

Chen IS, et al. Characterization of reticuloendotheliosis virus strain T DNA and isolation of a novel variant of reticuloendotheliosis virus strain T by molecular cloning. J. Virol. 40: 800-811, 1981. PubMed: 6275117

王經理

手機號：17280875617

QQ：1438578920
胡經理

手機號：13345964880

QQ：2438244627
周經理

手機號：17757487661

QQ：1296385441
于經理

手機號：18067160830

QQ：2088210172
沈經理

手機號：19548299266

QQ：2662369050
李經理

手機號：13626845108

QQ：972239479