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pH2L
pH2L
規(guī)格:
價格:
編號:B199411
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
pH2L
商品貨號
B199411
Designations
pH2L
Species
Homo sapiens, human
培養(yǎng)基
Depositors
M Fukuda
Vector
Construct size (kb): 3.680000066757202
Insert
DNA: cDNA
Insert lengths(kb): 0.7300000190734863
Tissue: erythroleukemic cell line
Gene product: glycophorin A (includes MN blood group)(glycophorin A) [MNS]
Insert Size (kb)
0.730
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.75; BamHI--3.8; HindIII--3.8; PstI--3.8; XbaI--3.8.
The insert includes the complete coding sequence, extending from 3 bp upstream of the initiation methionine to 276 bp downstream from the stop codon.
The nucleotide sequences encoding the NH2-terminal leader peptide and first 26 amino acids of GYPA and GYPB are nearly identical. There is also a very homologous region encoding the presumed membrane-spanning portion of the proteins.
This probe detects the same polymorphism as HGpA-3.
References

Siebert PD, Fukuda M. Isolation and characterization of human glycophorin A cDNA clones by a synthetic oligonucleotide approach: nucleotide sequence and mRNA structure. Proc. Natl. Acad. Sci. USA 83: 1665-1669, 1986. PubMed: 3456608

Minoru Fukuda, personal communication

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