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p3.4 [p3.4BHI]

規(guī)格:

價格:

編號:B199076

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

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產(chǎn)品名稱

p3.4 [p3.4BHI]

商品貨號

B199076

Designations

p3.4 [p3.4BHI]

Species

Homo sapiens, human

培養(yǎng)基

Depositors

RL White

Vector

Construct size (kb): 7.800000190734863

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749

Insert

DNA: genomic

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: IGHJ
Gene name: immunoglobulin heavy polypeptide, joining region
Contains complete coding sequence?: U
Chromosome: 14; Localization: 14 q32.33
Type of DNA: genomic
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 3.4
Cross references:

Insert lengths(kb): 3.400000095367432

Gene product: immunoglobulin heavy polypeptide, joining region [IGHJ]

Alleles: A1, A2, A3, A4

Insert Size (kb)

3.400

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--4.4, 3.4; BglII--6.4, 1.4; EcoRI--7.8; EcoRV--7.8; PstI--7.8.

This fragment originates 1.25 kb 5' to a BglII site just 5' to the J(H) sequences.

Subcloned from Ch28-6.

Enzymes(s) not detecting polymorphism: SstI, EcoRV, EcoRI, HaeIII, PvuII, PstI, TaqI, NdeII.

References

Silva AJ, et al. Characterization of a highly polymorphic region 5' to JH in the human immunoglobulin heavy chain. Nucleic Acids Res. 15: 3845-3857, 1987. PubMed: 2884636

Raymond L White, personal communication

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