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YIp31 [pRB22]
YIp31 [pRB22]
規格:
價格:
編號:B199024
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
YIp31 [pRB22]
商品貨號
B199024
Designations
YIp31 [pRB22]
Depositors
D Botstein
培養基
Biosafety Level
1
Vector Information
Size (kb): 5.5000000000000000
Vector: YIp31 (plasmid)
Construction: pBR322, Saccharomyces cerevisiae
Marker(s):URA3,ampR
Construct size (kb): 5.5
Features: marker(s): ampR, URA3
replicon: pMB1
Applications
YI-type (integrating) shuttle vector
integrating vector
shuttle vector
Comments
A general purpose integrating Saccharomyces cerevisiae/Escherichia coli shuttle vector.
This was constructed by cloning the 1.1 kb HindIII fragment containing URA3 into the HindIII site of pBR322. The 5' - 3' orientation of URA3 is clockwise.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030

Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863

Shipped
freeze-dried
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