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Hf32
Hf32
規(guī)格:
價格:
編號:B198763
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Hf32
商品貨號
B198763
Designations
Hf32
Species
Homo sapiens, human
培養(yǎng)基
Depositors
JC Myers
Vector
Construct size (kb): 6.5
Insert
DNA: cDNA
Insert lengths(kb): 2.099999904632568
Tissue: skin fibroblast
Gene product: collagen, type I, alpha 2 [COL1A2]
Alleles: A1, B1, K1, C1, A2, C2, K2, b2
Insert Size (kb)
2.100
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
The 5' EcoRI site was lost in cloning. The insert contains the following sites (in approximate kb from the 5' end): AvaI--0.3; PvuII--0.5, XhoI--0.75; AvaI--0.8; PstI--1.15; BglI-1.35; XbaI--1.55; SacI--1.85.
Restriction digests of the clone give the following sizes (kb): HindIII--6.5; EcoRI--6.5; PstI--4.6, 1.8; BamHI--6.5; SalI--6.5; HindIII/SstI--4.2, 1.2, 1.1.
This insert codes for 480 residues of the collagenous region. It also encodes most of the COOH-terminal propeptide.
This clone detects major mRNA species of about 5.1 and 4.6 kb in human skin fibroblasts and a minor transcript of about 4.4 kb.
References

Myers JC, et al. Cloning a cDNA for the pro-alpha2 chain of human type I collagen. Proc. Natl. Acad. Sci. USA 78: 3516-3520, 1981. PubMed: 6267597

Bernard MP, et al. Structure of a cDNA for the proalpha2 chain of human type I procollagen. Comparison with chick cDNA for proalpha2(I) identifies structurally conserved features of the protein and the gene. Biochemistry 22: 1139-1145, 1983. PubMed: 6687691

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