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plambda500 [p500]
plambda500 [p500]
規(guī)格:
價格:
編號:B198660
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
plambda500 [p500]
商品貨號
B198660
Designations
plambda500 [p500]
Species
Homo sapiens, human
培養(yǎng)基
Depositors
C Caskey, PI Patel
Vector
Construct size (kb): 10.25
Insert
DNA: genomic
Insert lengths(kb): 5.900000095367432
Gene product: hypoxanthine phosphoribosyltransferase pseudogene 2 [HPRTP2]
Alleles: A1, A2
Insert Size (kb)
5.900
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.1 kb doublet; HindIII--10.25; PstI--10.25; EcoRI/BamHI--5.6, 4.1, 1.25; EcoRI/HindIII--5.1, 4.4, 0.9.
The 0.9 kb EcoRI/HindIII fragment represents the HPRTP2 gene.
The insert includes a 5.0 kb EcoRI fragment and a 0.9 kb EcoRI/HindIII fragment, with the latter subcloned from Hulambda5.
The 3.8 kb constant band is from the X chromosome.
Enzyme(s) not detecting polymorphism: TaqI, PstI, BstNI, XbaI, SacI, PvuII, HindIII, HincII, StuII, MboI, EcoRV.
References

Weiffenbach B, et al. A genetic linkage map of human chromosome 5 with 60 RFLP loci. Genomics 10: 173-185, 1991. PubMed: 1675192

Patel PI, et al. Organization of the HPRT gene and related sequences in the human genome. Somatic Cell Mol. Genet. 10: 483-493, 1984. PubMed: 6089358

C. Thomas Caskey, personal communication

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