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TFTA1 [3721.95.161.4]
TFTA1 [3721.95.161.4]
規(guī)格:
價(jià)格:
編號(hào):B197988
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
TFTA1 [3721.95.161.4]
商品貨號(hào)
B197988
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with Sp2/5 myeloma cells (a subclone of Sp2/0-Ag14).
Genes Expressed
immunoglobulin; monoclonal antibody; against the A chain (A1 and A2) of the plant toxin ricin
Cellular Products
immunoglobulin; monoclonal antibody; against the A chain (A1 and A2) of the plant toxin ricin
Comments
Animals were immunized with ricin A chain.
Spleen cells were fused with Sp2/5 myeloma cells (a subclone of Sp2/0-Ag14).
The antibody does not cross-react with the ricin B chain.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1
Name of Depositor
R Fulton
Deposited As
mouse (B cell); mouse (myeloma)
References

Fulton RJ, et al. Purification of ricin A1, A2, and B chains and characterization of their toxicity. J. Biol. Chem. 261: 5314-5319, 1986. PubMed: 3957927

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