Promoters: Promoter for in vitro transcription T7 (phi10)
Construction: pSU18
Marker(s):ampR,cmlR
Construct size (kb): 4.144000053405762
Features: marker(s): cmlR promoter for in vitro transcription: T7 (phi10) replicon: p15A MCS: KpnI...SacI MluI MCS: MluI SacI...KpnI coding sequence: bla (ampR)
Applications
contains easily purifiable cassette(s) for construction omega - Ap
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--2.1, 2.0; EcoRI--2.1 (doublet); KpnI--2.15, 2.0; MluI--2.2, 2.0; SstI--2.15, 2.0.
Plasmid useful for generation of marked deletions and insertions in cloned DNA in vitro. Contains an omega - Ap element flanked by multiple cloning sites duplicated in inverted orientation.
The PstI and HincII sites in the bla gene have been removed.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718
