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pBS39 [BSY90]
pBS39 [BSY90]
規格:
價格:
編號:B196631
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pBS39 [BSY90]
商品貨號
B196631
Designations
pBS39 [BSY90]
GenBank Number

X03453

培養基
Species
Staphylococcus aureus subsp.aureus bacteriophage P1
Depositors
DuPont Merck Pharmaceutical Company, BL Sauer, DuPont Merck Pharmaceutical Company
Applications
in another host, produces protein recombinase, site-specific
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: unknown
Intact vector size:
Type of vector: plasmid
Vector end: SalI
Vector end: XhoI
Cloning sites:
Polylinker sites:
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, URA3
Cross references:
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: bacteriophage P1
Gene symbol: cre
Gene name: recombinase, site-specific
Contains complete coding sequence?: U
Type of DNA: genomic
Insert 5' end: XhoI
Insert 3' end: SalI
Insert size (kb): 1.2
Cross references: DNA Seq. Acc.: X03453
Insert lengths(kb): 1.200000047683716
Gene product: recombinase, site-specific [cre]
Target Gene: recombinase, site-specific
Insert Size (kb)
1.200
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): SalI/XhoI--4.1 (doublet), 1.5; SalI--9.6; XhoI--5.5, 4.1.
Permits inducible expression of cre in S. cerevisiae regulated by the GAL1 promoter.
The order of the major features in this plasmid is: ampR - GAL1 promoter - cre - URA3 - ARS1 - CEN4.
References

Sauer BL. Site-specific recombination of DNA in eukaryotic cells. US Patent 4,959,317 dated Sep 25 1990

Sauer B. Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae. Mol. Cell. Biol. 7: 2087-2096, 1987. PubMed: 3037344

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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