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RA-1

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價格:

編號:B196327

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

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凍干粉

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產品名稱

RA-1

商品貨號

B196327

Designations

RA-1

GenBank Number

D16217

培養基

Species

Homo sapiens, human

Applications

produces protein calpastatin

Vector

Construct size (kb): 5.919000148773193

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEX-4T-1
Intact vector size: 4.969
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI SmaI SalI XhoI NotI
Polylinker sites: EcoRI SmaI SalI XhoI NotI
Other unique sites: PstI mLuI BstEII ApaI BssHII EcoRV NarI BamHI
Construction: pGEX-2T
Host range: Escherichia coli
Features (with orientation and position when available):
other: thrombin protease cleavage site
MCS: EcoRI/.../NotI
marker(s): ampR, ->
replicon: pMB1
repressor gene: lacI, ->
promoter: tac, ->
coding sequence: S. japonicum glutathione S-transferase
Cross references:

Insert

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CAST
Gene name: calpastatin
Contains complete coding sequence?: N
Chromosome: 5; Localization: 5 q15-q21
Tissue: placenta
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 0.95
Cross references: DNA Seq. Acc.: D16217
Nucleotides 1-950 of the insert correspond to
nucleotides 1435-2384 of D16217.

Insert lengths(kb): 0.949999988079071

Tissue: placenta

Gene product: calpastatin [CAST]

Target Gene: calpastatin

Insert Size (kb)

0.950

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)

Comments

Restriction digests of the clone give the following sizes (kb): EcoRI--5.0, 1.0; BamHI--5.9; PstI--4.6, 1.35; HindIII--5.4, 0.5; EcoRI/PstI--4.2, 1.0, 0.7.

The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--0.13, 0.54; PstI--0.66.

This clone expresses a fusion protein of GST and calpastatin (domains 3 and 4 only). This truncated product also differs from calpastatin by a substitution (G->A) at nt 1937 of D16217, resulting in a glycine to glutamate substitution (G592E).

References

Despres N, et al. Detection and expression of a cDNA clone that encodes a polypeptide containing two inhibitory domains of human calpastatin and its recognition by rheumatoid arthritis sera. J. Clin. Invest. 95: 1891-1896, 1995. PubMed: 7706496

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