Comments
Restriction digests of the clone give the following sizes (kb): PstI--4.5, 4.2, 2.5, 2.0, 0.45; PvuII--5.8, 4.0, 1.75, 1.35, 0.54; EcoRI--14.0; HindIII--7.8, 4.4, 1.15, 0.72; BamHI--9.0, 2.4, 2.0, 1.2; BamHI+HindIII--4.4, 3.8, 2.4, 1.2 (doublet), 0.8;
ClaI--9.0, 5.6.
There is more than one SmaI site within the myc gene.
A 5.2 kb BamHI(5')/ClaI(3') fragment containing exons 2 and 3 has been used as a probe. The BamHI site is in intron 1 and the ClaI site is from the flanking pBR322-derived sequences.
This clone is incorrectly cited in U.S. Patent 4,736,866 as ATCC 39746.
The insert begins approximately 1.0 kb 5' of exon 1. It therefore contains the 2 promoters naturally preceding the unactivated gene.
Constructed by ligating a SmaI/EcoRI fragment from a genomic subclone of the gene to a SmaI/EcoRI fragment of pA9 (containing the glucocorticoid control region, MMTV promoter and cap site).
The 3' end of the myc sequence in this construct is the HindIII site approximately 1.0 kb 3' of the poly(A) addition site. The EcoRI site used in subcloning was derived from the vector (pBR322).
