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pSVLfur-mu

規格:

價格:

編號:B194265

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pSVLfur-mu

商品貨號

B194265

Designations

pSVLfur-mu

GenBank Number

L26489

培養基

Species

Mus musculus, mouse

Depositors

WJ Van De Ven

Applications

in another host, produces protein furin

Vector

Construct size (kb): 7.650000095367432

Insert

DNA: cDNA

Insert lengths(kb): 2.75

Gene product: furin [Fur]

Target Gene: furin

Insert Size (kb)

2.75

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--6.9, 0.8; EcoRI--2.7 (doublet), 2.2; KpnI--5.6, 1.45, 0.6; NcoI--3.6, 1.4, 0.9, 0.75, 0.45, 0.3, 0.25; PstI--5.2, 2.5.

The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--1.99; KpnI--0.15, 0.73; NcoI--0.36, 0.80, 1.66, 2.61; NotI--0.16; PstI--2.47.

The EcoRI insert site in pSVL was introduced in the SmaI site.

Classification

Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod

References

Van de Ven WJ, et al. Furin: the prototype mammalian subtilisin-like proprotein-processing enzyme. Endoproteolytic cleavage at paired basic residues of proproteins of the eukaryotic secretory pathway. Enzyme 45: 257-270, 1991. PubMed: 1843280

Creemers JW, et al. Life Sci. Adv. 11: 127-138, 1992.

Wim J Van De Ven, personal communication

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