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pWS11

規格:

價格:

編號:B193634

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pWS11

商品貨號

B193634

Designations

pWS11

GenBank Number

X14219

培養基

Species

Wound tumor virus

Depositors

DL Nuss

Vector

Construct size (kb): 3.990000009536743

Insert

DNA: cDNA

Insert lengths(kb): 1.129999995231628

Gene product: segment S11, capsomer protein P9

Insert Size (kb)

1.130

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

The insert contains internal EcoRI, BamHI, AccI and HincII sites.

Restriction digests of the clone give the following sizes (kb): BamHI/PstI--3.1, 0.86, 0.32; BamHI--3.1, 0.86, 0.32; PstI--3.1, 1.2; HindIII--4.2; SmaI--4.2.

Linearize the plasmid with HindIII for in vitro transcription.

This is a full-length clone of virus segment S11.

The cDNA ends were tailored to BamHI and PstI sites before cloning into the PstI site of pGEM-1.

Classification

Phytoreovirus

References

Xu ZK, et al. Assignment of wound tumor virus nonstructural polypeptides to cognate dsRNA genome segments by in vitro expression of tailored full-length cDNA clones. Virology 168: 73-78, 1989. PubMed: 2909991

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