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pWS10

規格:

價格:

編號:B191072

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pWS10

商品貨號

B191072

Designations

pWS10

GenBank Number

M24114

培養基

Species

Wound tumor virus

Depositors

DL Nuss

Vector

Construct size (kb): 4.039999961853027

Insert

DNA: cDNA

Insert lengths(kb): 1.169999957084656

Gene product: segment S10, Pns11 polypeptide

Insert Size (kb)

1.170

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments

HindIII cuts the insert twice, 7 bp apart.

The insert contains internal EcoRI, AvaI, SalI, AccI, HincII, PstI and HindIII sites.

Restriction digests of the clone give the following sizes (kb): BamHI--3.1, 1.2; EcoRI--3.1, 0.83, 0.41; HindIII--3.9, 0.38; PstI--3.4, 0.92; XbaI--4.3.

Linearize the plasmid with SphI for in vitro transcription.

This is a full-length clone of virus segment S10.

The cDNA ends were tailored to terminal BamHI sites before cloning into the BamHI site of pGEM-1.

Classification

Phytoreovirus

References

Xu ZK, et al. Assignment of wound tumor virus nonstructural polypeptides to cognate dsRNA genome segments by in vitro expression of tailored full-length cDNA clones. Virology 168: 73-78, 1989. PubMed: 2909991

Donald L Nuss, personal communication

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