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Bad KO SV40 MEF
Bad KO SV40 MEF
規格:
價格:
編號:B189276
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Bad KO SV40 MEF
商品貨號
B189276
Organism
Mus musculus, mouse
Tissue
embryonic fibroblast
培養基
Cell Type
fibroblast
Product Format
frozen
Morphology
fibroblast-like
Culture Properties
adherent
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
This cell line is useful to study molecular mechanism of cell apoptosis, BCL-2 family pathway, and BAD function.
Storage Conditions
liquid nitrogen vapor phase
Images
CRL-2909 Micrograph
Derivation
Cells are immortalized MEFs generated from BAD knockout mice.
Tumorigenic
no
Comments

Cells are immortalized MEFs (genotype: bad-/-), which were generated from BAD knockout mice. 

BAD is a pro-apoptotic BCL-2 family member (“BH3-only” subset) and is regulated by phosphorylation in response to growth/survival factors

This cell line is useful to study molecular mechanism of cell apoptosis, BCL-2 family pathway, and BAD function.

Complete Growth Medium
The base medium for this cell line is ATCC-formulated IMDM Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: 10 % Fetal Bovine Serum and 1x Non-essential amino acids.
Subculturing
Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 7 X 103 to 1.5 X 104 viable cells/cm2 is recommended.
  6. Incubate cultures at 37°C. At subculture, cell concentration is between 8 X 104 to 1.5 X 105 cells/cm2.
Subcultivation ratio: A subcultivation ratio of 1:6 to 1:12 is recommended.
Medium renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: 90% Fetal Bovine Serum and 10% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor
S Korsmeyer
References

Danial N, et al. BAD and glucokinase reside in a mitochondrial complex that integrates glycolysis and apoptosis. Nature 424 (6951) : 952-6, 2003 PubMed: 12931191

Cheng EH, et al. BCL-2, BCL-XL Sequester BH3 Domain-Only Molecules Preventing BAX- and BAK-Mediated Mitochondrial Apoptosis. Molecular Cell 8(3) : 705–711, 2001 PubMed: 11583631

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