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pFa6a-KANMX6-pADH1

規格:

價格:

編號:B187582

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

pFa6a-KANMX6-pADH1

商品貨號

B187582

Designations

pFa6a-KANMX6-pADH1

Depositors

DJ DeMarini

培養基

Biosafety Level

Host

Distribution host: Escherichia coli DH10B

Vector Information

Size (kb): 4.6479997634887700

DESCRIPTION OF VECTOR:
Intact vector size: 4.648
Type of vector: plasmid
Cloning sites: PacI PmeI BglII
Polylinker sites:
Construction: pCRTOPO2.1
Features (with orientation and position when available):

Vector: pFa6a-KANMX6-pADH1 (plasmid)

Construction: pCRTOPO2.1

Marker(s):ampR

Construct size (kb): 4.64799976348877

Applications

cloning vector

Comments

Restriction digests of the clone give the following sizes (kb): BglII--4.6; SalI/BglII--3.9, 0.7; EcoRI--4.6.

The marker and promoter are flanked by the F4 primer site (5') and the R6 primer site (3'). The size of the F4/R6 PCR product should be 2005 nt.

F4 primer sequence: 5'-(gene-specific sequence)GAATTCGAGCTCGTTTAAAC-3'. R6 primer sequence: 5'-(gene-specific sequence) CATTGTATATGAGATAGTTGA-3'.

Contains a functional truncated (700 nt) ADH promoter. The promoter is not down regulated during the exponential phase.

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Growth Conditions

Temperature: 37.0°C

References

DeMarini DJ, et al. Constitutive promoter modules for PCR-based gene modification in Saccharomyces cerevisiae. Yeast 18: 723-728, 2001. PubMed: 11378899